Effect of Ritonavir on the Pharmacodynamics of Gliclazide in Animal Models

نویسندگان

  • Shaik Mastan
  • Kilari Eswar Kumar
چکیده

The present study was performed to investigate the effect of ritonavir on the pharmacodynamics of gliclazide in rats (normal and diabetic) and rabbits to evaluate the safety and effectiveness of the combination. All blood samples were analyzed for blood glucose by the GOD/POD method and insulin in rabbit blood samples by the radioimmunoassay method. Ritonavir alone produced significant elevation in glucose and insulin levels. Gliclazide produced hypoglycemic/antidiabetic activity in normal and diabetic rats with peak activity at 2 h and 8 h and hypoglycemic activity in normal rabbits at 3 h. In combination, ritonavir significantly (P<0.05) enhanced the effect of gliclazide in rats and rabbits and this effect was more significant following multiple dose than single dose administration. Contrary to the theoretical expectation, this interaction between ritonavir and gliclazide appeared to be pharmacokinetic rather than pharmacodynamic. The combination may need dose adjustment and care should be taken when the combination is prescribed for clinical benefit in diabetic patients.

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تاریخ انتشار 2009